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Title: Neuroprotective effects of zinc and linoleic acid in ratmodel of parkinsonism induced with rotenone
Authors: Mbiydzenyuy, Ngala Elvis
Keywords: Sensorimotor assessment
Zinc and linoleic acid
Issue Date: Aug-2017
Publisher: Kampala International University, School of Health Sciences
Abstract: Introduction: Studies have investigated the neuroprotective effects of either zinc or linoleic acid but none has investigated the combined effects. Little is known about the behavioural effect of either zinc or linoleic acid or their combination in the management of Parkinson’s disease. Aim: This study was designed to investigate the neuroprotective effects of zinc and linoleic acid in rotenone-induced Parkinsonism in rats. Materials and Methods: Thirty six young adult female rats weighing 100-150 grams divided into six groups were used. Rats were induced with Parkinsonism by subcutaneous administration of rotenone (2.5mg/kg) once a day for seven consecutive days. The rats received DMSO/Olive oil or rotenone dissolved in (Dimethyl sulfoxide) DMSO/Olive oil. Groups III and IV received zinc (30mg/kg) or linoleic acid (150pJ/kg) while group V received a combination of both, two weeks prior to rotenone injection. Groups 11 and VI served as negative (rotenone group) and positive (Levodopa groups) controls respectively. Measurement and analysis of behavioural function in rats employed a battery of tests including elevated plus maze (EPM), open field test (OFT), novel scent and block tests. The oxidative stress levels were assessed by estimating Lipid peroxidation, Total Antioxidant Capacity, Superoxide Dismutase, reduced Glutathione, Glutathione Peroxidase and Catalase in the midbrain. Histological examination was done to assess structural changes in the midbrain Results: Rats receiving rotenone displayed bradykinesia and motor impairment in the OFT, anxiety, decrease in olfactory acuity and discrimination in EPM, FST and Novel Scent Test respectively. In addition, histological examination revealed that parkinsonian rat brains exhibited neuronal damage. There was a significant reduction in lipid peroxidation and improvement in the antioxidant status in intervention-treated groups. The significant increase in postural instability, impaired motor activity/coordination, increase anxiety and the decrease in rearing behaviour caused by rotenone induction was attenuated significantly by treatment with zinc and linoleic acid, but not their’combination. Conclusion: These results suggest that zinc and linoleic acid and their combination showed significant neuroprotective activity most likely due to the antioxidant effect
Description: A dissertation submitted to the Directorate of Postgraduate Studies and Research, in partial fulfillment of the requirements for the award of Master of Science degree in Physiology of Kampala International University
Appears in Collections:Masters of Science in Physiology

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